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Figure 6: Apoptosis detection using flow cytometry. The apoptosis of MCF-7 cells after combinational treatment was determined by flow cytometry using Annexin V/PI. A: A bar graph of flow cytometric analysis. B: Flow cytometric analysis of MCF-7 cells treated with each drug alone or combination drugs. *P<0.05 and ***P<0.001 compared with the control group. DCF: Diclofenac; PIP: Piperine; LIM: D-limonene. |
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