Figure 5: Effect of erastin, a ferroptosis inducer, on the viability, apoptosis, inflammation, and oxidative stress in NT2 cells. (A) The LDH levels in each group of cells were detected by an LDH kit. (B-C) Apoptosis was determined by flow cytometry. (D) The expression of apoptosis-related proteins was determined by Western blotting analysis. (E) The levels of inflammatory factors were determined by ELISA kit. (F) The levels of the oxidative stress markers were determined by assay kits. The data are expressed as mean±standard deviation and analyzed by ANOVA. ^***P<0.001 vs. the control group; ^###P<0.001 vs. the OGD group; ^&P<0.05, ^&&P<0.01, ^&&&P<0.001 vs. the OGD+Sal group.