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Figure 1: Effect of salidroside on the viability and apoptosis in NT2 cells. (A) The effect of different concentrations of salidroside on the viability of normal NT2 cells and (B) OGD-treated NT2 cells was determined using the CCK8 assay. (C) LDH levels in each group of cells were detected by the LDH kit. (D) Apoptosis was determined by flow cytometry. (E) The expression of apoptosis-related proteins was determined by Western blotting analysis. β-actin was used as an internal control. The data are expressed as mean±standard deviation and analyzed by ANOVA. ***P<0.001 vs. the control group; #P<0.05, ##P<0.01, ###P<0.001 vs. the OGD group. Sal: salidroside; OGD: oxygen and glucose deprivation; LDH: lactate dehydrogenase. |
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