Figure 6: Expression of apelin/APJ protein in the kidney tissue. (A) Western blotting of apelin and APJ protein levels. Quantification result of (B) APJ and (C) apelin in renal tissue. (D) Immunofluorescence of apelin in renal tissue (40×). (E) Quantitative fluorescence result of apelin. Siv/Sivelestat: PQ+sivelestat group; AH/AH2QDS: PQ+anthrahydroquinone-2,6-disulfonate (AH2QDS) group. Compared to the control group, **P<0.01; Compared with the PQ group, #P<0.05, ##P<0.01. The arrow shows the region where apelin protein fluoresce strongly.