Figure 3: Analysis of the phosphorylation profiles of kinases in A549 lung cancer cells. (A) The human phosphokinase array detected phosphorylated proteins in control, 20 μM THC- and 20 μM CBN-treated A549 lung cancer cells after an 18 h incubation. The bar graph shows the relative change in phosphorylated kinase proteins between THC-treated, CBN-treated, and untreated A549 lung cancer cells. CBN- and THC-treated cells show less phosphorylation of AKT (S473), glycogen synthase kinase 3 alpha/beta (GSK-3α/β), and endothelial nitric oxide synthase (eNOS). (B) Western blot analysis shows that THC and CBN at 20 μM inhibit the phosphorylation of AKT (S473), GSK-3α/β, and eNOS. β-Actin was used as a loading control.