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  Indian J Med Microbiol
 

Figure 2: THC and CBN induce apoptosis in A549 lung cancer cells. (A) Flow cytometry data shows the percentage of live, apoptotic, and necrotic A549 cells after 18 h incubation with the vehicle, or THC or CBN at 10 μM or 20 μM. (B) Flow cytometry analysis demonstrated that THC at 10 μM and 20 μM, and CBN at 20 μM, significantly increase apoptotic cells compared to the control (ANOVA, *P<0.05). In addition, 20 μM THC significantly increases the number of apoptotic A549 cells compared to 10 μM THC (ANOVA, ##P<0.001). (C) Cleaved PARP in A549 lung cancer cells following 10 μM and 20 μM THC or CBN treatment for 18 h was determined by Western blot analysis. PARP was used as a loading control.

Figure 2: THC and CBN induce apoptosis in A549 lung cancer cells. (A) Flow cytometry data shows the percentage of live, apoptotic, and necrotic A549 cells after 18 h incubation with the vehicle, or THC or CBN at 10 μM or 20 μM. (B) Flow cytometry analysis demonstrated that THC at 10 μM and 20 μM, and CBN at 20 μM, significantly increase apoptotic cells compared to the control (ANOVA, *<i>P</i><0.05). In addition, 20 μM THC significantly increases the number of apoptotic A549 cells compared to 10 μM THC (ANOVA, <sup>##</sup><i>P</i><0.001). (C) Cleaved PARP in A549 lung cancer cells following 10 μM and 20 μM THC or CBN treatment for 18 h was determined by Western blot analysis. PARP was used as a loading control.