Figure 4: Effect of co-treatment with SYR and Cu (II) on ROS generation (A) and mitochondrial membrane potential (MMP) (B) in HepG2 cells. ROS formation was determined by DCFH-DA and MMP was determined by rhodamine 123. The data are expressed as mean ± SD of at least three independent experiments and analyzed by one-way analysis of variance followed by Dunnett post-test. ***P < 0.001 compared with the control group. ###P < 0.001 compared with the Cu (II) group (250 μM).