Figure 5: Effect of dieckol on activation of the NF-κB signaling pathways in RANKL-induced RAW 264.7 cells. Cells were pre-treated with 25 μM dieckol for 2 h and then treated with RANKL for 5 min. (A) Protein expression was analyzed using Western blotting analysis. (B) Nuclear translocation of p65 in RANKLinduced RAW 264.7 cells. These images were observed with an anti-p65 and Alexa Fluor 488 goat anti-rabbit antibody using an LSM700 Zeiss confocal laser scanning microscope (magnification: 400 ×; scale bar: 20 μm). Nuclei were stained with Hoechst 33342. All results are expressed as mean ± standard deviation (SD) of at least three independent experiments. #P < 0.001 compared with the control group; *P < 0.001 compared with the RANKL-stimulated group.