Figure 4: Effect of dieckol on the expression of osteoclast-related transcriptional factors in RANKL-induced RAW 264.7 cells. (A) Protein expression was analyzed using Western blotting analysis. Nuclear translocation of (B) NFATc1, (C) c-Fos, and (D) c-Jun in RANKL-induced RAW 264.7 cells. These images were observed with an anti-NFATc1, anti-c-Fos, anti-c-Jun, Alexa Fluor 488 goat anti-mouse antibody, and Alexa Fluor 488 goat anti-rabbit antibody using an LSM700 Zeiss confocal laser scanning microscope (magnification: 400 ×; scale bar: 20 μm). Nuclei were stained with Hoechst 33342. All results are expressed as mean ± standard deviation (SD) of at least three independent experiments. #P < 0.001 compared with the control group; *P < 0.001 compared with the RANKL-stimulated group.