Figure 3: Effect of different concentrations of dieckol on TRAP activity and osteoclast-related factors in RANKL-induced RAW 264.7 cells. (A) TRAP activity was evaluated by TRAP staining (magnification: 100 ×; scale bar: 100 μm). Black arrows: TRAP-positive cells. (B) Quantitative results of TRAP activity. (C) The expression of calcitonin receptor (CTR) and TRAP protein was analyzed by Western blotting analysis. All results are expressed as mean ± SD of at least three independent experiments. #P < 0.001 compared with the control group; *P < 0.05, ***P < 0.001 compared with the RANKL-stimulated group.