Figure 5: SCEO activates mitochondrial apoptotic pathways and inhibits STAT3 phosphorylation in Eca109 cells. (A) Western blotting analysis of apoptosisassociated proteins in Eca109 cells treated with SCEO. (B-D) Relative levels of Bax, Bcl-2, Bcl-2/Bax ratio, cleaved caspase-3, cleaved caspase-9, PARP, and cleaved PARP. (E) Western blotting analysis of p-STAT3 and STAT3 in Eca109 cells treated with SCEO. (F) Relative levels of p-STAT3 (Tyr705) proteins. Data are presented as mean ± SD of three independent experiments. *P<0.05, **P<0.01 vs. control.