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Figure 4: SCEO induces Eca109 cell apoptosis. (A) Morphological analysis of Eca109 cells treated with SCEO. (B) Eca109 cells were treated with 7.5, 15, and 22.5 μg/mL of SCEO for 24 h. Apoptosis was assessed via flow cytometry after annexin V-FITC/propidium iodide (PI) staining. (C) Eca109 cells were treated with SCEO for 24 h, stained with annexin V-FITC/PI, and observed under a fluorescence microscope. |
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