Figure 1: Effect of CIEE on cell viabilities and morphological changes. A: HSC-T6 and Chang liver cells were incubated with CIEE at indicated concentrations for 24 h and cell viability was determined by MTT assay. B: TGF-β (5 ng/mL)-induced HSC-T6 cells were incubated with CIEE at indicated concentrations (0.1, 0.25, and 0.5 mg/mL) and cell proliferation was determined by MTT assay. C: Primary HSCs were cultured for 1 week and exposed to the CIEE (0.5 mg/ mL) for 24 h. Pictures were taken before and after 24 h treatment with CIEE. Black arrows represent the corresponding morphological changes (magnification was 100×). NS, not significant, #P<0.05, ###P<0.001 compared with control, *P<0.05 and **P<0.01, compared with TGF-β-induced HSC-T6 cell group. DMSO: Dimethyl sulfoxide; TGF-β: Transforming growth factor-beta; CIEE: Chrysanthemum indicum ethanol extract; HSC-T6: hepatic stellate cells-T6.