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  Indian J Med Microbiol
 

Figure 7: Effect of CFE on the LPS-induced nuclear factor-κB (NF-κB) P65 nuclear translocation. (A) RAW264.7 macrophages were pre-treated with CFE (200 μg/mL) for 24 h, and NF-κB P65 nuclear translocation was analyzed by immunofluorescence microscopy. Immunoreactivity of NF-κB P65 is shown in green, while the nuclei are stained with DAPI in blue. (B) Nuclear and cytoplasmic P65 levels were examined by Western blotting assays. (C) Ratios of nuclear P65/ cytoplasm (cyto) P65 were quantified by densitometry using ImageJ software. Data from three independent experiments are shown. ##P<0.01 vs. the control group. **P<0.01 vs. the LPS-treated group.

Figure 7: Effect of CFE on the LPS-induced nuclear factor-κB (NF-κB) P65 nuclear translocation. (A) RAW264.7 macrophages were pre-treated with CFE (200 μg/mL) for 24 h, and NF-κB P65 nuclear translocation was analyzed by immunofluorescence microscopy. Immunoreactivity of NF-κB P65 is shown in green, while the nuclei are stained with DAPI in blue. (B) Nuclear and cytoplasmic P65 levels were examined by Western blotting assays. (C) Ratios of nuclear P65/ cytoplasm (cyto) P65 were quantified by densitometry using ImageJ software. Data from three independent experiments are shown. <sup>##</sup><i>P</i><0.01 <i>vs</i>. the control group. **<i>P</i><0.01 <i>vs</i>. the LPS-treated group.