Figure 6: The PI3K/AKT/mTOR signaling pathway after DET treatment. HCT116 cells were treated with DET at different durations of incubation (6, 12 and 24 h). A representative Western blot of protein bands of AKT, phosphorylated (p)-AKT (Ser473), p-AKT (Thr308), p-PDK1, p-PTEN, p-mTOR, mTOR and RAPTOR is presented. GAPDH was used as loading control. The numbers above each band represent their respective mean fold change collectively from three separate experiments.