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  Indian J Med Microbiol
 

Figure 4: Oxidative stress in HCT116 cells induced by DET. Histogram (A) and bar chart (B) show intracellular ROS generation. Tert-butyl hydroperoxide (TBHP) served as positive control. ROS level (C), cell cytotoxicity (D), representative blots (E) and bar chart (F) of LC3A/B and p62 expressions, representative blots (G) and bar chart (H) of apoptosis-related protein expressions of DET-treated cells after NAC (ROS inhibitor) treatment. Data are expressed as mean ± SE of three separate experiments. *P<0.05, **P <0.05, #P <0.05 compared to the control, non-NAC treated group and DET-treated group, respectively.

Figure 4: Oxidative stress in HCT116 cells induced by DET. Histogram (A) and bar chart (B) show intracellular ROS generation. Tert-butyl hydroperoxide (TBHP) served as positive control. ROS level (C), cell cytotoxicity (D), representative blots (E) and bar chart (F) of LC3A/B and p62 expressions, representative blots (G) and bar chart (H) of apoptosis-related protein expressions of DET-treated cells after NAC (ROS inhibitor) treatment. Data are expressed as mean ± SE of three separate experiments. *P<0.05, **P <0.05, <sup>#</sup>P <0.05 compared to the control, non-NAC treated group and DET-treated group, respectively.