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  Indian J Med Microbiol
 

Figure 3: Autophagy-dependent apoptosis in HCT116 cells induced by deoxyelephantopin (DET). Cleaved caspase-3 and LC3A/B expressions in cells treated with 3-MA (autophagy inhibitor) (A, B) or z-VAD-FMK (apoptosis inhibitor) (C, D) before DET treatment. Acidic vesicular organelles in DET-treated cells, with or without z-VAD-FMK (E). Magnification = 400x. Relative protein expression after knockdown with three different siLC3 (F, G). LC3 and cleaved PARP expressions in siLC3-knockdown cells (H, I). GAPDH and β-actin were used as loading controls. Data are expressed as mean ± SE of three separate experiments. *P<0.05, #P<0.05 compared to the control and DET treatment group, respectively.

Figure 3: Autophagy-dependent apoptosis in HCT116 cells induced by deoxyelephantopin (DET). Cleaved caspase-3 and LC3A/B expressions in cells treated with 3-MA (autophagy inhibitor) (A, B) or z-VAD-FMK (apoptosis inhibitor) (C, D) before DET treatment. Acidic vesicular organelles in DET-treated cells, with or without z-VAD-FMK (E). Magnification = 400x. Relative protein expression after knockdown with three different siLC3 (F, G). LC3 and cleaved PARP expressions in siLC3-knockdown cells (H, I). GAPDH and β-actin were used as loading controls. Data are expressed as mean ± SE of three separate experiments. *P<0.05, #P<0.05 compared to the control and DET treatment group, respectively.