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  Indian J Med Microbiol
 

Figure 6: Hizikia fusiforme ethanol extracts (EHF)-induced apoptosis mediated by ROS-dependent pathways in PC3 cells. After pretreatment with 10 mM NAC for 1 h, 100 μg/mL EHF was added to the medium for 48 h. (A) Cells were dyed with JC-1 and then analyzed for MMP. (B) The expression of cytochrome c was examined in cytosolic and mitochondrial fractions. (C) The activity of caspase-3 was measured using ELISA kit. (D) Cell viability was measured by MTT assay. (E) Morphological changes to nuclei were observed. (F) The percentage of apoptotic cells was measured. *P<0.05 and ***P<0.001 when compared to untreated cells.

Figure 6: <i>Hizikia fusiforme</i> ethanol extracts (EHF)-induced apoptosis mediated by ROS-dependent pathways in PC3 cells. After pretreatment with 10 mM NAC for 1 h, 100 μg/mL EHF was added to the medium for 48 h. (A) Cells were dyed with JC-1 and then analyzed for MMP. (B) The expression of cytochrome c was examined in cytosolic and mitochondrial fractions. (C) The activity of caspase-3 was measured using ELISA kit. (D) Cell viability was measured by MTT assay. (E) Morphological changes to nuclei were observed. (F) The percentage of apoptotic cells was measured. *<i>P</i><0.05 and ***<i>P</i><0.001 when compared to untreated cells.