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  Indian J Med Microbiol
 

Figure 5: Accumulation of intracellular ROS after treatment with ethanol extracts of Hizikia fusiforme (EHF) in PC3 cells. (A) Cells were treated with 100 μg/ mL EHF for different times. After pretreatment with 10 mM N-acetyl-L-cysteine (NAC) for 1 h, 100 μg/mL EHF was added to the medium for another 1 h. ROS levels were visualized by flow cytometry (A and B) and fluorescence microscope (C) after 10 μM 5,6-carboxy-2',7'-dichlorodihydrofluorescein diacetate (DCF-DA) staining for 20 min.

Figure 5: Accumulation of intracellular ROS after treatment with ethanol extracts of <i>Hizikia fusiforme</i> (EHF) in PC3 cells. (A) Cells were treated with 100 μg/ mL EHF for different times. After pretreatment with 10 mM N-acetyl-L-cysteine (NAC) for 1 h, 100 μg/mL EHF was added to the medium for another 1 h. ROS levels were visualized by flow cytometry (A and B) and fluorescence microscope (C) after 10 μM 5,6-carboxy-2',7'-dichlorodihydrofluorescein diacetate (DCF-DA) staining for 20 min.