Figure 4: Induction of mitochondrial dysfunction and cytosolic release of cytochrome c by treatment with ethanol extracts of Hizikia fusiforme (EHF) in PC3 cells. (A) After 48 h treatment with EHF, cells were dyed with JC-1 and then changes of MMP were evaluated. ***P<0.001 compared to untreated cells. (B) Expression of cytochrome c by Western blot analysis with cytosolic and mitochondria fractions. Equal protein loading was confirmed by actin and cytochrome oxidase subunit VI (COX VI) in each protein extract, respectively.