Asian Pacific Journal of Tropical Biomedicine

ORIGINAL ARTICLE
Year
: 2022  |  Volume : 12  |  Issue : 8  |  Page : 357--366

Hepatoprotective effect of date palm fruit extract against doxorubicin intoxication in Wistar rats: In vivo and in silico studies


Ahmed M Fatani1, Othman A.S. Baothman2, Lobna S Shash3, Huda A Abuaraki4, Mustafa A Zeyadi2, Salman B Hosawi1, Hisham N Altayb2, Mohamed K Abo-Golayel5 
1 Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
2 Biochemistry Department, Faculty of Science; Microbial Toxicology & Natural Products Center, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
3 Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
4 Laboratory Animal Unit, King Fahad Medical Research Centre, King Abdulaziz University, Jeddah, Saudi Arabia
5 Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia; Ain Shams University Research Institute, Ain Shams University Hospitals, Ain Shams University, Cairo, Egypt

Correspondence Address:
Mohamed K Abo-Golayel
Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia; Ain Shams University Research Institute, Ain Shams University Hospitals, Ain Shams University, Cairo, Egypt

Objective: To investigate the prophylactic efficacy of date palm fruit extract against doxorubicin-induced hepatotoxicity in Wistar albino rats. Methods: The rats were equally and randomly assigned to 6 groups: group 1 (untreated control), group 2 and 3 given daily oral administration of prophylactic aqueous extract of date palm fruit at 0.75 and 1.5 mg/kg body weight, respectively, and group 4, 5 and 6 intraperitoneally injected with doxorubicin at 15 mg/kg on day 30. Rats in group 5 and 6 received daily oral administration of aqueous extract of date palm fruit at 0.75 and 1.5 mg/kg body weight, respectively, for 30 d. The phytochemicals identified by GC-MS analysis were analyzed using in silico study. Antioxidant enzymes, liver enzymatic, biochemical parameters and histopathological analysis were determined to evaluate hepatoprotective activity of date extract. Results: Aqueous extract of date palm fruit significantly mitigated doxorubicin-induced changes in activities of liver enzymes, reduced reactive oxygen species levels, and suppressed lipid peroxidation and DNA damage. Moreover, aqueous extract of date palm fruit reduced doxorubicin-induced hepatic lesions. Molecular docking studies showed that most compounds of aqueous extract of date palm fruit identified via GC-MS had good interaction with proteins of human pregnane X receptor, oxygenase-1, and CYP2C9. Conclusions: The aqueous extract of date palm fruit mitigates doxorubicin-mediated DNA damage and hepatotoxicity, and restores normal liver function and may be a promising agent against the deleterious effects of doxorubicin.


How to cite this article:
Fatani AM, Baothman OA, Shash LS, Abuaraki HA, Zeyadi MA, Hosawi SB, Altayb HN, Abo-Golayel MK. Hepatoprotective effect of date palm fruit extract against doxorubicin intoxication in Wistar rats: In vivo and in silico studies.Asian Pac J Trop Biomed 2022;12:357-366


How to cite this URL:
Fatani AM, Baothman OA, Shash LS, Abuaraki HA, Zeyadi MA, Hosawi SB, Altayb HN, Abo-Golayel MK. Hepatoprotective effect of date palm fruit extract against doxorubicin intoxication in Wistar rats: In vivo and in silico studies. Asian Pac J Trop Biomed [serial online] 2022 [cited 2022 Aug 12 ];12:357-366
Available from: https://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=8;spage=357;epage=366;aulast=Fatani;type=0