Asian Pacific Journal of Tropical Biomedicine

ORIGINAL ARTICLE
Year
: 2022  |  Volume : 12  |  Issue : 8  |  Page : 343--350

Human Wharton’s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model


Wahyu Widowati1, Teresa Liliana Wargasetia1, Fanny Rahardja1, Rimonta F Gunanegara1, Didik Priyandoko2, Marisca Evalina Gondokesumo3, Ervi Afifah4, Cahyaning Riski Wijayanti4, Rizal Rizal5 
1 Faculty of Medicine, Maranatha Christian University, Jl. Surya Sumantri no 65, Bandung 40164, Indonesia
2 Faculty of Mathematic and Natural Science Education, Indonesia University of Education, Jl. Dr. Setiabudi no 229, Bandung 40154, Indonesia
3 Faculty of Pharmacy, University of Surabaya, Universitas Surabaya, Jl. Raya Kalirungkut, Surabaya 60293, Indonesia
4 Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl. Babakan Jeruk 2 no 9, Bandung 40163, Indonesia
5 Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl. Babakan Jeruk 2 no 9, Bandung 40163; Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Jl. Margonda Raya, Depok 1642, Indonesia

Correspondence Address:
Wahyu Widowati
Faculty of Medicine, Maranatha Christian University, Jl. Surya Sumantri no 65, Bandung 40164
Indonesia

Objective: To evaluate the potential effect of human Wharton’s jelly mesenchymal stem cells (hWJMSCs) on acute respiratory distress syndrome in lipopolysaccharide (LPS)-induced rats. Methods: The hWJMSCs (5×104/mL, 5×105/mL, 5×106/mL) were administered to rats on day 1 and day 8 after being induced by LPS (5 mg/kg body weight). TNF-α levels in the lung and IL- 18 and IL-1β levels in the serum were measured using ELISA. In addition, caspase-1 expression in lung tissues was quantified using qRT-PCR, and NF-κB and IL-6 expressions were assessed using immunohistochemistry. Results: The hWJMSCs decreased TNF-α levels in the lung and plasma IL-18 and IL-1β levels. Moreover, the hWJMSCs downregulated the expressions of caspase-1, IL-6, and NF-κB in lung tissues. Conclusions: The hWJMSCs can decrease inflammatory markers of acute respiratory distress syndrome in a rat model and may be further investigated for the treatment of acute respiratory distress syndrome.


How to cite this article:
Widowati W, Wargasetia TL, Rahardja F, Gunanegara RF, Priyandoko D, Gondokesumo ME, Afifah E, Wijayanti CR, Rizal R. Human Wharton’s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model.Asian Pac J Trop Biomed 2022;12:343-350


How to cite this URL:
Widowati W, Wargasetia TL, Rahardja F, Gunanegara RF, Priyandoko D, Gondokesumo ME, Afifah E, Wijayanti CR, Rizal R. Human Wharton’s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model. Asian Pac J Trop Biomed [serial online] 2022 [cited 2022 Aug 18 ];12:343-350
Available from: https://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=8;spage=343;epage=350;aulast=Widowati;type=0