Asian Pacific Journal of Tropical Biomedicine

ORIGINAL ARTICLE
Year
: 2022  |  Volume : 12  |  Issue : 1  |  Page : 39--46

Piperine suppresses growth and migration of human breast cancer cells through attenuation of Rac1 expression


Benjaporn Buranrat1, Mutita Junking2 
1 Faculty of Medicine, Mahasarakham University, Muang District, Maha Sarakham 44000, Thailand
2 Division of Molecular Medicine, Research Department, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand

Correspondence Address:
Benjaporn Buranrat
Faculty of Medicine, Mahasarakham University, Muang District, Maha Sarakham 44000
Thailand

Objective: To investigate the effect of piperine on human breast cancer cells. Methods: The effect of piperine on proliferation and migration of human breast cancer cells, MCF-7 and MDA-MB-231, was investigated using colony formation assays, wound healing assays, Matrigel migration assays, flow cytometry, RT-qPCR, and Western blotting assays. Results: Piperine inhibited the growth of MCF-7 and MDA-MB-231 cells and suppressed colony formation. Cell reduction at the G0/ G1 phase and cell arrest at the G2/M phase were observed in breast cancer cells. However, the significant effect was only demonstrated in MDA-MB-231 cells. Moreover, cancer cell migration was suppressed by piperine at low concentration. RT-qPCR and Western blotting assays showed that piperine downregulated Rac1 gene and protein expression. Conclusions: Piperine could inhibit growth and migration of breast cancer cells by reducing Rac1 gene and protein expression.


How to cite this article:
Buranrat B, Junking M. Piperine suppresses growth and migration of human breast cancer cells through attenuation of Rac1 expression.Asian Pac J Trop Biomed 2022;12:39-46


How to cite this URL:
Buranrat B, Junking M. Piperine suppresses growth and migration of human breast cancer cells through attenuation of Rac1 expression. Asian Pac J Trop Biomed [serial online] 2022 [cited 2022 Jan 28 ];12:39-46
Available from: https://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=1;spage=39;epage=46;aulast=Buranrat;type=0