Asian Pacific Journal of Tropical Biomedicine

ORIGINAL ARTICLE
Year
: 2022  |  Volume : 12  |  Issue : 11  |  Page : 475--482

Acanthus leucostachyus leaf extracts promote excision wound healing in mice


Deepjyoti Dev, Ashish Sarkar, Bishnupada Roy 
 Parasitology and Toxicology Laboratory, Department of Zoology, North-Eastern Hill University, Shillong-793022, Meghalaya, India

Correspondence Address:
Bishnupada Roy
Parasitology and Toxicology Laboratory, Department of Zoology, North-Eastern Hill University, Shillong-793022, Meghalaya
India

Objective: To evaluate the in vivo wound healing activity of Acanthus leucostachyus leaf extracts using an excision wound model in mice. Methods: Mice were divided into two groups of six animals in each group: the control group and the Acanthus leucostachyus extract-treated group. Healing potential was evaluated by determination of physical parameters (contraction rate, epithelialization period, and tensile strength), biochemical parameters (protein, DNA, and hydroxyproline content), the expression of growth factor and proinflammatory cytokines, as well as histological and ultrastructural observations. Results: Treatment with Acanthus leucostachyus leaf extracts markedly increased the rate of wound contraction, tensile strength, the concentrations of protein, DNA, and hydroxyproline, and the expression of growth factor, as well as promoted epithelialization, compared to the control. In addition, Acanthus leucostachyus leaf extracts significantly reduced the expression of pro-inflammatory cytokines. Histological and ultrastructural studies revealed the presence of thicker epithelial layer and smoother surface topography in the extract-treated group compared to the control. Conclusions: Acanthus leucostachyus leaf extracts show potent wound-healing activity and can be used as a wound healing agent.


How to cite this article:
Dev D, Sarkar A, Roy B. Acanthus leucostachyus leaf extracts promote excision wound healing in mice.Asian Pac J Trop Biomed 2022;12:475-482


How to cite this URL:
Dev D, Sarkar A, Roy B. Acanthus leucostachyus leaf extracts promote excision wound healing in mice. Asian Pac J Trop Biomed [serial online] 2022 [cited 2022 Dec 7 ];12:475-482
Available from: https://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=11;spage=475;epage=482;aulast=Dev;type=0