Asian Pacific Journal of Tropical Biomedicine

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 11  |  Issue : 11  |  Page : 481--490

Crotalaria ferruginea extract attenuates lipopolysaccharide-induced acute lung injury in mice by inhibiting MAPK/NF-κB signaling pathways


Wei Pan1, Li-Ping Meng2, Jie Su3, Zheng-Biao Yang5, Wei-Feng Du3, Zhi-Wei Xu3, Yun-Xiang Chen5, Sheng Zhang5, Feng Xie5, Cong Xu5, Hong-Zhong Yang5, Wei-Hong Ge3 
1 Department of Pharmacology, College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053; State Key Laboratory of Safety Evaluation for New Drugs, Hangzhou Medical College, Hangzhou 310013, China
2 Department of Surgical Nursing, The First Affiliated Hospital of Hainan Medical University, Haikou 570102, China
3 Department of Pharmacology, College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China

Correspondence Address:
Hong-Zhong Yang
State Key Laboratory of Safety Evaluation for New Drugs, Hangzhou Medical College, Hangzhou 310013
China
Wei-Hong Ge
Department of Pharmacology, College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053
China

Objective: To evaluate the anti-inflammatory activity of Crotalaria ferruginea extract (CFE) and its mechanism. Methods: An intratracheal lipopolysaccharide (LPS) instillation-induced acute lung injury (ALI) model was used to study the anti-inflammatory activity of CFE in vivo. The LPS-induced shock model was used to analyze the effect of CFE on survival. LPS-stimulated RAW264.7 cell model was used to investigate the anti-inflammatory activity of CFE in vitro and the effects on mitogen-activated protein kinase (MAPK) or nuclear factor-κB (NF-κB) signaling pathways. Results: CFE administration decreased the number of inflammatory cells, reduced the levels of tumor necrosis factor-α (TNF-a), monocyte chemotactic protein-1 (MCP-1), interleukin-6 (IL-6), and interferon-γ, and diminished protein content in the bronchoalveolar lavage fluid of mice. CFE also reduced lung wet-to-dry weight ratio, myeloperoxidase, and lung tissue pathological injury. CFE pre-administration improved the survival rate of mice challenged with a lethal dose of LPS. CFE reduced LPS-activated RAW264.7 cells to produce nitric oxide, TNF-α, MCP-1, and IL-6. Furthermore, CFE inhibited nuclear translocation and phosphorylation of NF-κB P65, extracellular signal-regulated kinase, c-Jun N-terminal kinases, and P38 MAPKs. Conclusions: CFE exhibits potent anti-inflammatory activity in LPS-induced ALI mice, LPS-shock mice, and RAW264.7 cells, and its mechanism may be associated with the inhibition of NF-κB and MAPK signaling pathways. Crotalaria ferruginea may be a useful therapeutic drug for the treatment of ALI and other respiratory inflammations.


How to cite this article:
Pan W, Meng LP, Su J, Yang ZB, Du WF, Xu ZW, Chen YX, Zhang S, Xie F, Xu C, Yang HZ, Ge WH. Crotalaria ferruginea extract attenuates lipopolysaccharide-induced acute lung injury in mice by inhibiting MAPK/NF-κB signaling pathways.Asian Pac J Trop Biomed 2021;11:481-490


How to cite this URL:
Pan W, Meng LP, Su J, Yang ZB, Du WF, Xu ZW, Chen YX, Zhang S, Xie F, Xu C, Yang HZ, Ge WH. Crotalaria ferruginea extract attenuates lipopolysaccharide-induced acute lung injury in mice by inhibiting MAPK/NF-κB signaling pathways. Asian Pac J Trop Biomed [serial online] 2021 [cited 2021 Dec 3 ];11:481-490
Available from: https://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=11;spage=481;epage=490;aulast=Pan;type=0