|
Hepatoprotective effect of date palm fruit extract against doxorubicin intoxication in Wistar rats: In vivo and in silico studies
Ahmed M Fatani1, Othman A.S. Baothman2, Lobna S Shash3, Huda A Abuaraki4, Mustafa A Zeyadi2, Salman B Hosawi1, Hisham N Altayb2, Mohamed K Abo-Golayel5
1 Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
2 Biochemistry Department, Faculty of Science; Microbial Toxicology & Natural Products Center, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
3 Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
4 Laboratory Animal Unit, King Fahad Medical Research Centre, King Abdulaziz University, Jeddah, Saudi Arabia
5 Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia; Ain Shams University Research Institute, Ain Shams University Hospitals, Ain Shams University, Cairo, Egypt
Correspondence Address:
Mohamed K Abo-Golayel
Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia; Ain Shams University Research Institute, Ain Shams University Hospitals, Ain Shams University, Cairo, Egypt
 Source of Support: The study was supported by King Abdulaziz City for Science and Technology (KACST) under grant number (1-18-03-009-0057), Conflict of Interest: None
DOI: 10.4103/2221-1691.350184
|
|
Objective: To investigate the prophylactic efficacy of date palm fruit extract against doxorubicin-induced hepatotoxicity in Wistar albino rats.
Methods: The rats were equally and randomly assigned to 6 groups: group 1 (untreated control), group 2 and 3 given daily oral administration of prophylactic aqueous extract of date palm fruit at 0.75 and 1.5 mg/kg body weight, respectively, and group 4, 5 and 6 intraperitoneally injected with doxorubicin at 15 mg/kg on day 30. Rats in group 5 and 6 received daily oral administration of aqueous extract of date palm fruit at 0.75 and 1.5 mg/kg body weight, respectively, for 30 d. The phytochemicals identified by GC-MS analysis were analyzed using in silico study. Antioxidant enzymes, liver enzymatic, biochemical parameters and histopathological analysis were determined to evaluate hepatoprotective activity of date extract.
Results: Aqueous extract of date palm fruit significantly mitigated doxorubicin-induced changes in activities of liver enzymes, reduced reactive oxygen species levels, and suppressed lipid peroxidation and DNA damage. Moreover, aqueous extract of date palm fruit reduced doxorubicin-induced hepatic lesions. Molecular docking studies showed that most compounds of aqueous extract of date palm fruit identified via GC-MS had good interaction with proteins of human pregnane X receptor, oxygenase-1, and CYP2C9.
Conclusions: The aqueous extract of date palm fruit mitigates doxorubicin-mediated DNA damage and hepatotoxicity, and restores normal liver function and may be a promising agent against the deleterious effects of doxorubicin.
|
Next article
