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REVIEW ARTICLE
Year : 2022  |  Volume : 12  |  Issue : 7  |  Page : 279-289

Anticancer drug screening of natural products: In vitro cytotoxicity assays, techniques, and challenges


Faculty of Pharmacy, Sanata Dharma University, Paingan, Maguwoharjo, Depok, Sleman, Yogyakarta 55282, Indonesia

Correspondence Address:
Agustina Setiawati
Faculty of Pharmacy, Sanata Dharma University, Paingan, Maguwoharjo, Depok, Sleman, Yogyakarta 55282
Indonesia
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Source of Support: This work was supported by the Internal Research Grant of Sanata Dharma University No. 007/Penel./LPPM-USD/II/2022, Conflict of Interest: None


DOI: 10.4103/2221-1691.350176

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Natural products include several diverse compounds that have been found to be effective against cancer. Discovering anticancer compounds in nature is a multistep and complex process that requires pre-clinical and clinical studies. Only a few of the available natural products are used to treat cancer since most of them have very high complexity and low bioavailability. Therefore, the process of anticancer drug discovery requires a straightforward and effective method to assess anticancer activity using in vitro assays. This review summarizes various cell-based assays and techniques used to measure cell viability, migration, and apoptosis, focusing in particular on the principles, mechanisms, advantages, and disadvantages of each assay to provide a preliminary platform for cancer drug discovery.


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