Protein extract of kenaf seed exhibits anticoagulant, antiplatelet and antioxidant activities
Sujatha M Hanumegowda1, Chandramma Srinivasa2, Ashwini Shivaiah2, Manjula M Venkatappa1, Ramesha Hanumanthappa2, Rajesh Rangappa3, Ramesh K Laxmaiah4, Sathisha J Gonchigar1, Devaraja Sannaningaiah2
1 Department of Biochemistry Jnansahydri, Kuvempu University, Shankarghatta-577451, Shivamogga, Karnataka, India
2 Department of Studies and Research in Biochemistry and Centre for Bioscience and Innovation, Tumkur University, Tumkur, Karnataka, India
3 Liveon Biolabs Private Limited, Tumkur-572106, India
4 Department of Food Science and Nutrition, Maharani’s Science College for Women, Mysore, India
Department of Studies and Research in Biochemistry and Centre for Bioscience and Innovation, Tumkur University, Tumkur, Karnataka
Sathisha J Gonchigar
Department of Biochemistry Jnansahydri, Kuvempu University, Shankarghatta-577451, Shivamogga, Karnataka
Source of Support: This work is supported by Vision Group of Science and Technology,
Government of Karnataka, India (VGST/CISEE/2012-13/282, dated
March 16th 2013)., Conflict of Interest: None
Objective: To explore the anticoagulant, antiplatelet and antioxidant activities of protein extract of kenaf seed (PEKS).
Methods: Sodium dodecyl sulphate polyacrylamide gel electrophoresis and reverse-phase high-performance liquid chromatography techniques were employed for protein characterization. Antioxidant activity of PEKS was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The protective effect of PEKS on sodium nitrite (NaNO2) induced oxidative stress was evaluated using the in vitro red blood cell model, while the effect of PEKS on diclofenac-induced oxidative stress was examined in vivo in rats. Platelet-rich plasma and platelet-poor plasma were used for anticoagulant and antiplatelet activities of PEKS.
Results: PEKS revealed similar protein bands on SDS-PAGE under reduced and non-reduced conditions. Several acidic proteins were present in native PAGE. PEKS showed antioxidant properties by scavenging DPPH with an IC50 of 24.58 μg. PEKS exhibited a protective effect on NaNO2 induced oxidative stress in red blood cells by restoring the activity of stress markers. In addition, PEKS alleviated diclofenac-induced tissue damage of the liver, kidney, and small intestine. PEKS showed an anticoagulant effect in both in vivo and in vitro experiments by enhancing normal clotting time. PEKS did not affect prothrombin time but increase activated partial thromboplastin time. Furthermore, PEKS inhibited adenosine diphosphate and epinephrine-induced platelet aggregation.
Conclusions: PEKS protects tissues from oxidative stress and exhibits antithrombotic activity.