| ORIGINAL ARTICLE |
|
| Year : 2022 | Volume
: 12
| Issue : 12 | Page : 504-511 |
|
|
Voacanga grandifolia (Miq.) Rolfe protects against alcohol-induced liver toxicity in rats
Lal Chand Pal1, Shivankar Agrawal2, Arti Gautam3
1 Pharmacology Division, CSIR-National Botanical Research Institute, Lucknow 226001, Uttar Pradesh, India
2 Department of Phytochemistry, ICMR-National Institute of Traditional Medicine, Nehru Nagar, Belagavi 590010, Karnataka, India
3 Pharmacology Division, CSIR-National Botanical Research Institute, Lucknow 226001; Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Naini, Prayagraj 211007, Uttar Pradesh, India
Correspondence Address:
Lal Chand Pal
Pharmacology Division, CSIR-National Botanical Research Institute, Lucknow 226001, Uttar Pradesh
India
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/2221-1691.363876
|
|
|
Objective: To evaluate the ethanol extract of Voacanga grandifolia for hepatoprotective and antioxidant potential against ethanolinduced liver toxicity in rats.
Methods: Sprague-Dawley rats were administered ethanol (7 g/kg) and then treated with 100 and 200 mg/kg of Voacanga grandifolia extract. The phytochemical constituents and antioxidant potential of Voacanga grandifolia extract were evaluated by GC-MS and in vitro antioxidant assays. Biochemical indicators for liver damage and proapoptotic and antiapoptotic gene expression were determined using biochemical kits, ELISA, and qRT-PCR, respectively. Additionally, histopathological study of the liver was performed.
Results: GC-MS identified propanoic acid, meso-erythritol, D-pinitol, myo-inositol, and hexadecanoic acid in Voacanga grandifolia extract. Voacanga grandifolia extract (100 and 200 mg/kg) increased the concentration of enzymatic antioxidants while diminishing the levels of inflammatory cytokines and biochemical indicators. qRT-PCR assay showed that Voacanga grandifolia extracts upregulated antiapoptotic gene expression while downregulating pro-apoptotic gene expression. Furthermore, the plant extract improved the hepatic architecture of ethanol-intoxicated rats.
Conclusions: Voacanga grandifolia extract demonstrates hepatoprotective activity against alcohol-induced liver injury in rats and could be a potential hepatoprotective agent. |
|
|
|
| [FULL TEXT] [PDF]* |
|
 |
|
