Lentinula edodes extract inhibits matrix metalloproteinase expression and increases type I procollagen expression via the p38 MAPK/c-Fos signaling pathway in ultraviolet A and B-irradiated HaCaT keratinocytes

Jung Im Lee; Jung Hwan Oh; Fatih Karadeniz; So Young Park; Hye Ran Kim; Hyun Jin Jo; Kyung Im Jung; Byung-Jin Jeon; Chang-Suk Kong

doi:10.4103/2221-1691.310203

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ORIGINAL ARTICLE

Year : 2021 \| Volume : 11 \| Issue : 4 \| Page : 164-173

Lentinula edodes extract inhibits matrix metalloproteinase expression and increases type I procollagen expression via the p38 MAPK/c-Fos signaling pathway in ultraviolet A and B-irradiated HaCaT keratinocytes Jung Im Lee¹, Jung Hwan Oh¹, Fatih Karadeniz¹, So Young Park², Hye Ran Kim², Hyun Jin Jo², Kyung Im Jung², Byung-Jin Jeon³, Chang-Suk Kong⁴ ¹ Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, Busan 46958, Korea ² Department of Food and Nutrition, College of Medical and Life Sciences, Silla University, Busan 46958, Korea ³ Ocean Fisheries and Bio Center, Busan technopark, Busan 46048, Korea ⁴ Marine Biotechnology Center for Pharmaceuticals and Foods; Department of Food and Nutrition, College of Medical and Life Sciences, Silla University, Busan 46958, Korea Correspondence Address: Chang-Suk Kong Marine Biotechnology Center for Pharmaceuticals and Foods; Department of Food and Nutrition, College of Medical and Life Sciences, Silla University, Busan 46958 Korea Source of Support: This research was supported by the Ministry of Trade, Industry & Energy, Korea Institute for Advancement of Technology and Busan Institute For Regional Program Evaluation through the Encouragement Program for the Social and Economic Innovation Growth (project number, P0008724), Conflict of Interest: None DOI: 10.4103/2221-1691.310203

Objective: To determine the effect of Lentinula edodes extract on ultraviolet (UV) A and UVB-induced changes in matrix metalloproteinase (MMP) and type I procollagen expression using human immortalized HaCaT keratinocytes. Methods: Lentinula edodes ethanol extract (LEE) was obtained by extraction with 80% ethanol for 4 h at 80 °C. Effect of LEE on UV-induced alteration on the expression and production of MMPs and type I procollagen in keratinocytes was investigated using ELISA, RT-PCR, and Western blotting assay. To determine the underlying mechanism of LEE-mediated effects, mitogen-activated protein kinase (MAPK) and activator protein 1 signaling pathways were analysed by Western blotting assay. Results: LEE significantly inhibited the expression of MMP-1 and MMP-9 and increased the expression of type I procollagen in UVA and UVB-irradiated HaCaT keratinocytes. The phosphorylation levels of p38 were significantly inhibited by LEE whereas it did not affect c-Jun N-terminal kinase and extracellular signal-regulated kinase phosphorylation. Suppression of p38 phosphorylation was also accompanied by downregulation of UVA and UVB-induced increase in c-Fos. Conclusions: LEE effectively inhibits the expression of MMP-1 and MMP-9 and increases type I procollagen production through the p38 MAPK/c-Fos signaling pathway in UVA and UVB-irradiated HaCaT keratinocytes. This findings suggest that Lentinula edodes may be developed as a cosmetic material to suppress UV exposuremediated skin aging.



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Lentinula edodes; Ultraviolet; Matrix metalloproteinases; Type I procollagen; HaCaT

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