Impact Factor 2020 : 1.545 (@Clarivate Analytics)
  • Users Online: 386
  • Print this page
  • Email this page
Year : 2021  |  Volume : 11  |  Issue : 2  |  Page : 66-73

Corchorus olitorius aqueous extract attenuates quorum sensing-regulated virulence factor production and biofilm formation

1 Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
2 University Malaysia Kelantan, Campus Jeli, 17600, Kelantan, Malaysia
3 Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, 44519 Zagazig, Egypt

Correspondence Address:
Hanan M Al-Yousef
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh
Saudi Arabia
Perwez Alam
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh
Saudi Arabia
Login to access the Email id

Source of Support: This research project was supported by a grant from the “Research Center of the Female Scientific and Medical Colleges”, Deanship of Scientific Research, King Saud University, Conflict of Interest: None

DOI: 10.4103/2221-1691.303605

Rights and Permissions

Objective: To investigate the effect of Corchorus olitorius aqueous fraction (COAF) on quorum sensing (QS)-regulated virulence factors and biofilm formation in Pseudomonas aeruginosa (PAO1). Methods: The preliminary screening of the anti-QS effect of COAF was performed by evaluating the anti-pathogenic activity against Chromobacterium violaceum CV026 biosensor strain. Next, the inhibitory effects of COAF on QS-regulated pyocyanin production, proteolytic and elastolytic activities, swarming motility, and biofilm formation were evaluated in PAO1. Results: The results showed that the treatment of COAF significantly decreased the biofilm biomass, attenuated virulence factors, and inhibited swarming motility of PAO1 without affecting the growth of the bacteria in a dose-dependent manner. COAF at 2 000 μg/mL significantly decreased Las B elastase activity in PAO1 culture, exopolysaccharide production, swarming motility, pyocyanin level, and biomass of PAO1 by 55% (P<0.05), 60% (P<0.01), 61% (P<0.01), 65%(P<0.01) and 73% (P<0.01), respectively. In addition, the production of violacein was decreased by 62% (P<0.01) with the treatment of a high dose of COAF. Conclusions: These findings indicate that COAF can be a potential source of anti-QS agents.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded237    
    Comments [Add]    
    Cited by others 1    

Recommend this journal