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ORIGINAL ARTICLE
Year : 2021  |  Volume : 11  |  Issue : 11  |  Page : 500-509

Chrysanthemum indicum ethanol extract attenuates hepatic stellate cell activation in vitro and thioacetamide-induced hepatofibrosis in rats


1 Department of Applied Life Science, Graduate School of Konkuk University, 268 Chungwon-daero, Chungju-si, Chungbuk, 27478; Scholar Foxtrot Co., LTD, 501, SinGwan-dong 5F, Jongam-ro 36-gil, Seongbuk-gu, Seoul, 02796, Republic of Korea
2 Department of Biotechnology, College of Biomedical and Health Sciences, Konkuk University, 268 Chungwon-daero, Chungju-si, Chungbuk, 27478, Republic of Korea
3 Department of Food Science and Engineering, Seowon University, Cheongju, Chungbuk 28674, Republic of Korea
4 Food One Corp, 127, Sincheoksandan 5-ro, Deoksan-eup, Jincheon-gun, Chungbuk 27850, Republic of Korea
5 Department of Applied Life Science, Graduate School of Konkuk University; Department of Biotechnology, College of Biomedical and Health Sciences; Research Institute and College of Biomedical & Health Science, Konkuk University, 268 Chungwon-daero, Chungju-si, Chungbuk, 27478, Republic of Korea

Correspondence Address:
MinDong Song
Department of Applied Life Science, Graduate School of Konkuk University; Department of Biotechnology, College of Biomedical and Health Sciences; Research Institute and College of Biomedical & Health Science, Konkuk University, 268 Chungwon-daero, Chungju-si, Chungbuk, 27478
Republic of Korea
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2221-1691.328057

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Objective: To investigate the antifibrotic effects of Chrysanthemum indicum ethanol extract (CIEE) against activated hepatic stellate cells (HSC) and thioacetamide (TAA)-induced hepatofibrosis in rats. Methods: Cell viability and proliferation of HSC-T6 cells were measured using MTT assay. Primary HSCs were used to study morphology. TAA (200 mg/kg) was used to induced hepatic fibrosis in rats. CIEE (100 and 500 mg/kg) and silymarin (50 mg/kg) were administered orally. Liver functions including alanine transaminase, aspartate transaminase, glutathione, and hydroxyproline levels were measured using commercial kits. Liver sections and fibrotic biomarker expression were measured using hematoxylin and eosin staining and real-time polymerase chain reaction. Results: In vitro study revealed that CIEE (0.1, 0.25, and 0.5 mg/mL) inhibited the proliferation of activated HSCs exposed to transforming growth factor (TGF)-β and restored the activated primary HSC morphology. In in vivo studies, TAA-induced increase in liver/body weight ratio (5.46 ± 0.26) was significantly reduced (4.13 ± 0.22) by CIEE (P<0.05 at 500 mg/kg). CIEE (100 and 500 mg/kg) improved the liver functions by significantly attenuating changes in alanine transaminase, aspartate transaminase, glutathione, and hydroxyproline levels (P<0.05). Further, CIEE (100 and 500 mg/kg) ameliorated the histological changes in liver tissue and TGF-β expression significantly (P<0.05) in TAA-induced rats. Conclusions: CIEE significantly protects against TAA-induced liver damage in rats and can be used in the treatment of liver fibrosis.


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