Impact Factor 2020 : 1.545 (@Clarivate Analytics)
  • Users Online: 205
  • Print this page
  • Email this page
ORIGINAL ARTICLE
Year : 2021  |  Volume : 11  |  Issue : 11  |  Page : 481-490

Crotalaria ferruginea extract attenuates lipopolysaccharide-induced acute lung injury in mice by inhibiting MAPK/NF-κB signaling pathways


1 Department of Pharmacology, College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053; State Key Laboratory of Safety Evaluation for New Drugs, Hangzhou Medical College, Hangzhou 310013, China
2 Department of Surgical Nursing, The First Affiliated Hospital of Hainan Medical University, Haikou 570102, China
3 Department of Pharmacology, College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China
4 State Key Laboratory of Safety Evaluation for New Drugs, Hangzhou Medical College, Hangzhou 310013, China

Correspondence Address:
Hong-Zhong Yang
State Key Laboratory of Safety Evaluation for New Drugs, Hangzhou Medical College, Hangzhou 310013
China
Wei-Hong Ge
Department of Pharmacology, College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053
China
Login to access the Email id

Source of Support: This work was supported by the Natural Science Foundation of Zhejiang province (Grant LQ19H280009); Special Projects of Zhejiang Academy of Medical Sciences (Grant CA1918D-04, CA1903Q-04); Medical Health Science and Technology Project of Zhejiang Provincial Health Commission (Grant 2020384536)., Conflict of Interest: None


DOI: 10.4103/2221-1691.328055

Get Permissions

Objective: To evaluate the anti-inflammatory activity of Crotalaria ferruginea extract (CFE) and its mechanism. Methods: An intratracheal lipopolysaccharide (LPS) instillation-induced acute lung injury (ALI) model was used to study the anti-inflammatory activity of CFE in vivo. The LPS-induced shock model was used to analyze the effect of CFE on survival. LPS-stimulated RAW264.7 cell model was used to investigate the anti-inflammatory activity of CFE in vitro and the effects on mitogen-activated protein kinase (MAPK) or nuclear factor-κB (NF-κB) signaling pathways. Results: CFE administration decreased the number of inflammatory cells, reduced the levels of tumor necrosis factor-α (TNF-a), monocyte chemotactic protein-1 (MCP-1), interleukin-6 (IL-6), and interferon-γ, and diminished protein content in the bronchoalveolar lavage fluid of mice. CFE also reduced lung wet-to-dry weight ratio, myeloperoxidase, and lung tissue pathological injury. CFE pre-administration improved the survival rate of mice challenged with a lethal dose of LPS. CFE reduced LPS-activated RAW264.7 cells to produce nitric oxide, TNF-α, MCP-1, and IL-6. Furthermore, CFE inhibited nuclear translocation and phosphorylation of NF-κB P65, extracellular signal-regulated kinase, c-Jun N-terminal kinases, and P38 MAPKs. Conclusions: CFE exhibits potent anti-inflammatory activity in LPS-induced ALI mice, LPS-shock mice, and RAW264.7 cells, and its mechanism may be associated with the inhibition of NF-κB and MAPK signaling pathways. Crotalaria ferruginea may be a useful therapeutic drug for the treatment of ALI and other respiratory inflammations.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed602    
    Printed0    
    Emailed0    
    PDF Downloaded103    
    Comments [Add]    

Recommend this journal