| ORIGINAL ARTICLE |
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| Year : 2020 | Volume
: 10
| Issue : 7 | Page : 308-315 |
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Ferruginol alleviates inflammation in dextran sulfate sodium-induced colitis in mice through inhibiting COX-2, MMP-9 and NF-κB signaling
Xiao-Yan Zhu1, Chun-Ling Zhang2, Yukiat Lin3, Min-Yan Dang3
1 Department of Gastroenterology, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013, Shandong Province, China
2 Department of Emergency, Taian City Central Hospital, Taian, 271000, Shandong Province, China
3 Innoscience Research Sdn Bhd, Subang Jaya, 47650, Selangor, Malaysia
Correspondence Address:
Min-Yan Dang
Innoscience Research Sdn Bhd, Subang Jaya, 47650, Selangor
Malaysia
 Source of Support: None, Conflict of Interest: None  | 4 |
DOI: 10.4103/2221-1691.284945
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Objective: To assess the anti-inflammatory efficacy of ferruginol on dextran sulfate sodium (DSS) stimulated ulcerative colitis mice.
Methods: Ulcerative colitis was induced in C57BL/6J mice by administering 2% of DSS through drinking water for 7 d. The mice in the treatment group were treated with DAA+50 mg/kg/day ferruginol orally. In the positive control group, sulfasalazine (50 mg/kg/day) was used alongside with DSS. After induction, the bodyweight, character of stool and feces occult blood were recorded daily, the disease activity index was calculated, and the colon length, colon weight, and spleen weight were recorded. The myeloperoxidase activity was assayed by spectrophotometry. Interleukin (IL)-6, IL-1β, and tumor necrosis factor-a were determined by ELISA method, and nuclear factor-κB, cyclooxygenase-2, matrix metalloproteinases-9, and inducible nitric oxide synthase by Western blotting assays.
Results: Ferruginol significantly increased the bodyweight, colon weight, colon length, and decreased disease activity index and spleen weight. It exhibited anti-inflammatory activity against DSS induced ulcerative colitis in mice by reducing the activities of myeloperoxidase, tumor necrosis factor-α, nuclear factor-κβ, IL-1β, cyclooxygenase-2, matrix metalloproteinases-9, IL-6, and inducible nitric oxide synthase.
Conclusions: Ferruginol could be used to treat ulcerative colitis by attenuating the inflammation in colon cells and maintaining colonic mucosal barrier integrity.
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