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Prediction of T cell and B cell epitopes of the 22-, 47-, 56-, and 58-kDa proteins of Orientia tsutsugamushi
Li-Na Niu1, Ting-Ting Fu2, Man-Ling Chen3, Yu-Ying Dong2, Jin-Chun Tu4, Zi-Hao Wang2, Si-Qi Wang2, Xuan Zhao2, Nai-Xu Hou2, Qian Chen5, Qiang Wu6
1 Laboratory of Tropical Biomedicine and Biotechnology, School of Tropical Medicine and Laboratory Medicine, Hainan Medical University; Department of Pathogen Biology, School of Basic Medicine and Life Science, Hainan Medical University; Hainan Medical University-University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Haikou, Hainan 571199, China
2 Laboratory of Tropical Biomedicine and Biotechnology, School of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou, Hainan 571199, China
3 Department of Gynecology, the First Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570102, China
4 State Key Laboratory of Marine Resource Utilization in South China Sea, College of Material science and Engineering, Hainan University, Haikou, Hainan 570228, China
5 Department of Laboratory, the First Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570102, China
6 Laboratory of Tropical Biomedicine and Biotechnology, School of Tropical Medicine and Laboratory Medicine; Key Laboratory of Emergency and Trauma of Ministry of Education & Research Unit of Island Emergency Medicine of Chinese Academy of Medical Sciences, Hainan Medical University, Haikou, Hainan 571199, China
Correspondence Address:
Qian Chen
Department of Laboratory, the First Affiliated Hospital of Hainan Medical University, Haikou Hainan 570102
China
Qiang Wu
Laboratory of Tropical Biomedicine and Biotechnology, School of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou, Hainan 571199
China
 Source of Support: None, Conflict of Interest: None  | 5 |
DOI: 10.4103/2221-1691.269526
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Objective: To predict B cell and T cell epitopes of 22-kDa, 47-kDa, 56-kDa and 58-kDa proteins.
Methods: The sequences of 22-kDa, 47-kDa, 56-kDa and 58-kDa proteins which were derived from Orientia tsutsugamushi were analyzed by SOPMA, DNAstar, Bcepred, ABCpred, NetMHC, NetMHC II and IEDB. The 58-kDa tertiary structure model was built by MODELLER9.17.
Results: The 22-kDa B-cell epitopes were located at positions 194-200, 20-26 and 143-154, whereas the T-cell epitopes were located at positions 154-174, 95-107, 17-25 and 57-65. The 47-kDa protein B-cell epitopes were at positions 413-434, 150-161 and 283-322, whereas the T-cell epitopes were located at positions 129-147, 259-267, 412-420 and 80-88. The 56-kDa protein B-cell epitopes were at positions 167-173, 410-419 and 101-108, whereas the T-cell epitopes were located at positions 88-104, 429-439, 232-240 and 194-202. The 58-kDa protein B-cell epitopes were at positions 312-317, 540-548 and 35-55, whereas the T-cell epitopes were located at positions 415-434, 66-84 and 214-230.
Conclusions: We identified candidate epitopes of 22-kDa, 47-kDa, 56-kDa and 58- kDa proteins from Orientia tsutsugamushi. In the case of 58-kDa, the dominant antigen is displayed on tertiary structure by homology modeling. Our findings will help target additional recombinant antigens with strong specificity, high sensitivity, and stable expression and will aid in their isolation and purification.
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